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  • 产品名称：dsGreen Gel Staining Solution, 10000×

  • 产品型号：20010
  • 产品厂商：其它品牌
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订购货号 产品名称及规格 价格（￥） 20010 dsGreen Gel Staining Solution, 10000×, 1 mL 2340.00
详情介绍：
订购货号 产品名称及规格 价格（￥）
20010 dsGreen Gel Staining Solution, 10000×, 1 mL 2340.00

 

SYBR Green I是一种敏感的dsDNA结合染料，可以用于日常琼脂糖和聚丙烯酰胺凝胶的DNA检测。
不同于Ethidium，SYBR Green I对双链DNA有高度选择性，更低的毒性，更高的灵敏度。


溴化乙锭（Ethidium Bromide）和SYBR Green I的对比

 

Feature

Ethidium Bromide

SYBR Green I

Fluorescence

Red (615 nm)

Green (524 nm)

Excitation maximum

302 nm

454 nm

Excitation light source

UV only

Blue light or UV

Sensitivity

2 ng / band(dsDNA)
100 ng / band (RNA)

0.08 ng / band (dsDNA)
1-2 ng / band (oligonucleotides)

Health hazard

High

Low

 

推荐手册：
SYBR Green I DNA凝胶染色
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
 

 

General properties

 

Appearance:

orange solution

Quality control:

NMR 1H (95%) and 13C, TLC, functional testing

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

MSDS:

Download

Spectral properties

 

Excitation maximum, nm:

454

Extinction coefficient at excitation maximum, Lmol-1cm-1:

73000

Emission maximum, nm:

524

Fluorescence quantum yield:

0.8

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